Journal: Cell reports

Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis

doi: 10.1016/j.celrep.2021.109347

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human PPIA Taqman probe Hs04194521_s1 , ThermoFisher , 4331182.

Techniques: Recombinant, Lysis, TUNEL Assay, RNAscope, Positive Control, Negative Control, Staining, Transfection, Purification, cDNA Synthesis, SYBR Green Assay, Knock-Out, Microarray, Knockdown, Control, Software

Journal: bioRxiv

Article Title: Optimisation of Xenium automated in situ sequencing for PAXgene-fixed tissue samples

doi: 10.1101/2025.02.11.637091

Figure Lengend Snippet: Narrowing down potential pre-treatment approaches using RNAscope smFISH as a proxy readout. (A) A total of 20 pre- treatment regimes were trialled using an RNAscope smFISH readout. (B-C) RNAscope staining of replicate sections of two mouse cerebellum tissue samples (from different animals) following aforementioned pre-treatments. Ubc RNA staining shown in green, DAPI-stained nuclei in blue. All full-tissue section images shown to the same scale (scale bar 1 mm); all inset images shown to the same scale (scale bar 50 µm).

Article Snippet: Tyramide signal amplification with Opal 520, Opal 570, and Opal 650 (Akoya Biosciences) was used to develop three RNAscope probe channels ( Polr2a , Opal 650; Ppib , Opal 570; Ubc , Opal 520).

Techniques: RNAscope, Staining

Journal: Cancer cell

Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis

doi: 10.1016/j.ccell.2019.12.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RNAScope Positive Control Probe- Hs-PPIB , ACD Bio , Cat. # 313901.

Techniques: Produced, Virus, Subcloning, Recombinant, Membrane, Western Blot, Autoradiography, RNA Extraction, Reverse Transcription, SYBR Green Assay, Sample Prep, Chromatin Immunoprecipitation, cDNA Synthesis, TA Cloning, Sequencing, RNAscope, Cell Culture, Control, DNA Methylation Assay, shRNA, Negative Control, Positive Control, Mutagenesis, Software

Journal: Cell reports

Article Title: Widespread sex-dimorphism across single-cell transcriptomes of adult African turquoise killifish tissues

doi: 10.1016/j.celrep.2023.113237

Figure Lengend Snippet: Key resource table

Article Snippet: RNAscope custom probe Nf-ppib-C2 , Advanced Cell Diagnostics , 563391-C2.

Techniques: Recombinant, Modification, RNAscope, Staining, Quantitation Assay, Sensitive Assay, Multiplex Assay, cDNA Synthesis, Control, Microscopy, Software, Cell Counting, Transferring, Sample Prep